PDF | Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted. PDF | Gerbera jamesonii (gerbera) is an important cut-flower in the global floricultural industry. Micropropagation is the main system used to. PDF | In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L-1 BAP and mg .
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Percentage of callus and root formation per explants produced on MS medium supplemented with different hormones and concentration after 8 weeks in culture. Petioles and leaves obtained from aseptic young plantlets formed from the seedlings were used as source of explants.
MICROPROPAGATION OF GERBERA | ICAR-Indian Institute of Horticultural Research
In the present study, experiments were conducted to investigate organogenesis from various sources of Gerbera explants. Principles of Plant Biotechnology. The most important aspect in plant tissue culture is the capability of cultured cells and tissues to regenerate into complete plants. Combination of auxin and cytokinin induces micropropzgation formation of adventitious shoots and roots.
Micropropagation of Gerbera (Gerbera jamesonii Bolus).
Cytokinin alone in the culture medium induces shoot formation in many plants. Rancillac and Nourrisseau improved the performance of micropropagated strawberry plants by decreasing the cytokinin concentration and limiting the number of subcultures. High efficiency adventitious shoot bud formation and plant regeneration from leaf explants of Dianthus chinensis L. They are planted outdoors in full sun and useful as cut flowers, pot plant and also bedding plant. Growth Optimization and Organogenesis of Gerbera jamesonii Bolus ex.
High concentrations of cytokinin in culture medium were found to be unsuitable for shoot formation from leaf or petiole explants in some ornamentals. Most papers in the literature have focused on testing the influence of different types and combinations of plant growth regulators with the aim of improving the regeneration and multiplication stage of one or few cultivars.
The regenerated plants were observed to be normal. Organogenesis and histogenesis of adventitious organ induced on leaf blade segments of Begonia elatior hybrids Begonia x hiemalis in tissue culture.
Relatively, in this experiment, callus formation was optimum when leaf explant was cultured on MS medium supplemented with 1. Various explants have been used for direct shoot formation.
Root explants were found to be non-regenerative in all experiments conducted.
Epigenetic variations in micropropagated gerbera are frequently observed only with high concentrations of cytokinins in the culture medium but somaclonal variation is rare. Pakistan Journal of Biological Sciences, Percentage of callus and root formation per explants produced on MS medium supplemented with different hormones and concentration after 8 weeks in culture formed in these experiments were mainly green.
Plantlets were transferred to soil and maintained in culture room for weeks for adaptation process before being transferred to field environment.
The present study showed that only petiole explant produced optimum results for shoot formation. However, white, creamy friable callus was obtained when explants were cultured on MS medium supplemented with 2, 4-D alone.
The effect of cytokinin type and concentration and the number of subcultures on the multiplication rate of some decorative plants. Thus, improvements of plant quality need to be studied and more research needs to be done.
One of the most important techniques in plant improvements is via micro propagation. Slow-growing endogenous bacteria are one of the most important problems in gerbera micropropagation but require more studies on control and prevention. Springer-Verlag, New York, pp: Auxins, indoleamines and ion channels in the regeneration of Echinacea purpurea L.
This research was funded by Vote F Grant No. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis.
Plant propagation by tissue culture technique is mainly aimed to produce plants with very high multiplication rates. However, regeneration of root was not observed when explants mivropropagation cultured on MS medium supplemented with BAP and 2, 4-D.
Gerbera jamesonii Bolus ex. Genotype is one of microlropagation most influential factors on the response of gerbera in vitro. Different types of auxin and cytokinin combinations were used in order to obtain complete regeneration of Gerbera in vitro. Kluwer Academic Publishers, Dordrecht, pp: Adventitious organogenesis or shoot formation is a preferred system as it enables to retain the clonal fidelity since many ornamental species are cultivars that are propagated for one or more unique characteristics Kantia and Kothari,