The standard orcinol test for estimating RNA is modified and developed as a specific method for the determination of RNA in the presence of DNA and proteins. ESTIMATION OF RNA BY (RIBOSE SUGAR) BY ORCINOL METHOD · Estimation of DNA (Deoxy Ribose) by Diphenyl Amine Method. Biochemistry and. Estimation of RNA (Orcinol method). Principle: This is a general reaction for pentoses. Acid hydrolysis of RNA releases the ribose sugar and this in the presence.
|Published (Last):||9 August 2005|
|PDF File Size:||3.54 Mb|
|ePub File Size:||16.86 Mb|
|Price:||Free* [*Free Regsitration Required]|
The reaction in the early stage was found to take place by the 2-deoxy-D-ribose in DNA molecules, and the color intensity was proportional to the amount of DNA. This constancy provides a simple method for an evaluation of purity of DNA preparations and it is applicable to the simultaneous estimation of DNA and RNA in the mixture of them.
The mechanism of DNA-orcinol reaction is discussed. Most users should sign in with their email address.
If you originally registered with a username please meghod that to sign in. To purchase short term access, please sign in to your Oxford Academic account above. Don’t already have an Oxford Academic account?
Orange Estimation Of RNA By Orcinol Method Teaching Kit | ID:
Oxford University Press is a department of the University of Oxford. It furthers the University’s objective of excellence in research, scholarship, and education by publishing worldwide. Sign In or Create an Account.
Close mobile estimztion navigation Article navigation.
Methodology for Nucleic Acids
You do not currently have access to this article. You could not be signed in. Sign In Forgot password?
Don’t have an account? Japanese Biochemical Society members Sign in via society site. Sign in via your Institution Sign in.
Purchase Subscription prices and ordering Short-term Access To purchase short term access, please sign in to your Oxford Academic account above. This article is also available for rental through DeepDyve.
A modified orcinol test for the specific determination of RNA.
Email alerts New issue alert. Receive exclusive offers and updates from Oxford Academic. Related articles in Web of Science Google Scholar. Citing articles via Dstimation of Science Latest Most Read Most Cited Fine epitope mapping of a human disulfide-stabilized diabody against fibroblast growth factor A novel splicing variant of small nucleolar RNA host gene 4 is a podocyte-selective non-coding RNA upregulated in response to puromycin aminonucleoside-induced podocyte injury.